Methods and compositions for the production of stably transforme

Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses

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4351721, 435410, 435424, 935 52, 935 55, 935 67, 935 85, 800205, 800DIG56, 47 58, 47DIG1, C12N 1500, C12N 1582, A01H 106, A01H 400

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active

059196750

ABSTRACT:
This invention relates to a method for the production of stable, genetically transformed, Zea mays plants that comprise an exogenous DNA expressing phosphinothricin acetyl transferase to impart resistance to phosphinothricin in said plants. The method further comprises transforming said Zea mays plants with a second gene and further still wherein said second gene imparts insect resistance, such as the expression of a Bacillus thuringiensis toxin gene. The method for achieving stable transformation includes tissue culture methods and media, methods for the bombardment of recipient cells with said DNA's, and methods of regenerating fertile plants from transformed cells. The invention also pertains to a method of breeding said transgenic Zea mays plants with a either a non-transgenic plant or a transgenic plant, and the obtention of fertile transgenic plants and seed therefrom.

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