Materials and method for immobilizing biologically active substa

Details Materials and method for immobilizing biologically active substa Materials and method for immobilizing biologically active substa

1983-01-04

1989-05-09

Warden, Robert J.

Chemistry: molecular biology and microbiology

Carrier-bound or immobilized enzyme or microbial cell;...

Enzyme or microbial cell is immobilized on or in an organic...

435174, 435181, C12N 1102, C12N 1100, C12N 1106

Patent

active

048289965

ABSTRACT:
Materials and a method of their preparation are described, for use in immobilizing soluble, biologically active compounds. The material for immobilizing includes a hydrophobic, water insoluble substrate having a coating of a mixture of different amphiphilic lipoid compounds thereon. The lipoid compounds are oriented so that the hydrophilic portions are distal to the substrate and free to bond with water soluble proteins and carbohydrates.

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patent: 4066512 (1978-01-01), Lai et al.
patent: 4151049 (1979-04-01), Janata
Shaltiel, "Hydrophobic Chromatography", in Methods In Enzymology, vol. 34, New York, Academic Press, 1974, pp. 126-140.
The Merck Index, Windholz et al (ed.), Tend Edit., Rahway, N.J., Merck & Co., 1983, pp. 352, 353 and 760.
Folch et al, "A Simple Method for the Isolation and Purification of Total Lipides from Animal Tissues", Journal Biological Chemistry, vol. 226 (1957), pp. 497-509.
Streitwieser, Jr. et al, Introduction to Organic Chemistry, New York, MacMillan Publishing, 1976, pp. 849-850.

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