Accurate quantitation of RNA and DNA by competetitive polymerase

Details Accurate quantitation of RNA and DNA by competetitive polymerase Accurate quantitation of RNA and DNA by competetitive polymerase

1989-08-31

1993-05-25

Moskowitz, Margaret

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 91, 536 2433, 536 243, 935 77, 935 78, C12Q 168, C07H 1700, C12P 1934, C12N 1500

Patent

active

052139611

ABSTRACT:
A process for quantitating nucleic acids species in a sample is described which comprises co-amplification of a competitive template with the sample template wherein the competitive template utilizes primers identical to those utilized by the sample template and wherein the competitive template is distinguishable from the sample template.

REFERENCES:
patent: 4683202 (1987-07-01), Mullis
Chelly et al, Nature, 333 pp. 858-860 (1988).
Kwok et al, Nature, vol. 339, May 18, 1989, pp. 237-238.
Rappolee, D. A. et al., Science 241:708-712 (1988).
Chelly, J. et al., Nature 333:858-860 (1988).
Higuchi, R. et al., Nucl. Acids Res. 16(15):7351-7369 (1988).
G. Gilliland et al., UCLA Symposia on Molecular and Cellular Biology; The Polymerase Chain Reaction: Methodology and Applicatikons, Apr. 3-24, 1989, Keystone, Colo., pub'd in J. Cell Biochem., Supp 13E, p. 270 (1989).

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