Immunoreactive reagent particles having tracer, receptor molecul

Details Immunoreactive reagent particles having tracer, receptor molecul Immunoreactive reagent particles having tracer, receptor molecul

1987-09-18

1989-03-14

Marantz, Sidney

Chemistry: analytical and immunological testing

Involving an insoluble carrier for immobilizing immunochemicals

Carrier is organic

435 7, 435 36, 436534, 436547, 436825, G01N 33546, G01N 33547, G01N 33569

Patent

active

048124141

ABSTRACT:
A reagent useful in the determination of an immunoreactive species comprises water-insoluble particles having tracer molecules associated therewith. Bound to the outer surface of the particles are: (i) receptor molecules which are reactive with the species, and (ii) molecules of a protein having a pI less than about 6, and which protein is not reactive with either the species or the receptor molecules. The weight ratio of the receptor molecules to the low pI protein molecules is from about 100:1 to about 1:10. This reagent is useful in agglutination and other immunological reactions. A method of preparing the reagent includes providing a suspension of the particles and contacting them with the receptor molecules and low pI protein so as to attach both to the particles. The protein is present in the suspension in an amount such that substantially all of it is attached to the particles. The reagent thus prepared can be used, for example, in agglutination assays for the determination of a multivalent immunoreactive species, such as Streptococcus A antigen.

REFERENCES:
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patent: 4618576 (1986-10-01), Rosenstein
patent: 4656144 (1987-04-01), Hosaka
patent: 4677080 (1987-06-01), Finkelstein
patent: 4713350 (1987-12-01), Siegel
L. F. Fieser et al., "Advanced Organic Chemistry", p. 1048, Reinhold, New York, 1961.
Johnson et al., Gene. Anal. Techn. 1 (1984) pp. 3-8.

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