Fluorescent dyes and biological and analytical uses thereof

Details Fluorescent dyes and biological and analytical uses thereof Fluorescent dyes and biological and analytical uses thereof

1986-01-31

1989-03-14

Rosen, Sam

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

435 7, 435 29, 435 34, 436800, 422 56, 568369, G01N 3354, C12Q 100

Patent

active

048123935

ABSTRACT:
Substituted 4-oxo-4H-benz-[d,e] anthracenes are fluorescent dyes which are useful in biomedical studies and analytical determinations. They are particularly useful in assays for living organisms, e.g. microorganisms, carried out at a pH of 9 or less. For these determinations, the fluorescent dyes can be attached to reducible compounds which will release the dye upon reduction. Alternatively, these dyes can be used in assays for hydrolytic enzymes or biological cells containing these enzymes. For these determinations, the dyes are attached to blocking groups.

REFERENCES:
patent: 4036859 (1977-07-01), Ribaldone et al.
patent: 4296043 (1981-10-01), Schroeder
patent: 4591569 (1986-05-01), Wagner et al.
Cooke et al., Aust. J. Chem., 11, pp. 230-235 (1958).
Cooke et al., Aust. J. Chem. 28, pp. 1053-1057 (1975).
Cooke et al., Aust. J. Chem. 30, pp. 2241-2247 (1977).
Cooke et al., Aust. J. Chem., 32 pp. 1841-1847 (1979).
Chaffer et al., Aust. J. Chem. 34 pp. 587-598 (1981).
Haddon--Chem. Abstr. vol. 98 (1983) page 53364b.

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