Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of...
Patent
1996-01-05
1998-11-17
Wax, Robert A.
Chemistry: molecular biology and microbiology
Animal cell, per se ; composition thereof; process of...
536 235, 435 691, 4353201, C12N 522, C12N 1512, C12N 1586
Patent
active
058375368
ABSTRACT:
A DNA sequence for a human mdr1 gene, which encodes p-glycoprotein, wherein at least one base in a splice region of the DNA encoding p-glycoprotein is changed. Such a mutation prevents truncation of the p-glycoprotein upon expression thereof. There is also provided a method of identifying cells which express the human mdr1 gene in a cell population that has been transduced with an expression vehicle including a human mdr1 gene. The method comprises contacting the cell population with a staining material, such as rhodamine 123, and identifying cells which express the human mdr1 gene based on differentiation in color among the cells of the cell population. This method has allowed identification of retroviral producer clones facilitate mdr gene transfer into primary cells. Repopulating hematopoietic stem cells have been genetically engineered with the human mdr1 gene.
REFERENCES:
patent: 4912039 (1990-03-01), Riordan
patent: 5166059 (1992-11-01), Pastan et al.
patent: 5175099 (1992-12-01), Wills
patent: 5192553 (1993-03-01), Boyse et al.
patent: 5198344 (1993-03-01), Croop et al.
patent: 5206352 (1993-04-01), Roninson et al.
Safa, et al., Proc. Nat. Acad. Sci., vol. 87, pp. 7225-7229 (1990).
Weinstein, et al. "Multidrug Resistance Gene Product (P-Glycoprotein) in Normal Tissue and Tumors," Advances in Pathology and Laboratory Medicine, Mosby Yearbook, Inc. (1991).
Schinkel, et al., Cancer Research, vol. 51, pp. 2628-2635 (May 15, 1991).
Devine, et al., Proc. Nat. Acad. Sci., vol. 89, pp. 4565-4568 (May 1992).
Pastan, et al., FASEB J., vol. 5, pp. 2523-2528 (1991).
Chaudhary, et al., Cell, vol. 68, pp. 85-94 (Jul. 12, 1991).
Devine et al. Mar. 5, 1991. J. Biol. Chem. 266(7):4545-4555.
Chaudhary et al. 1991. Cell 66:85-94.
Pastan et al. 1991. The FASEB J. 5:2523-2528.
Aebi et al. 1986. Cell 47:555-565.
Ohshima et al. 1987. J. Mol. Biol. 195:247-259.
McDonagh Kevin T.
Nienhuis Arthur
Tolstoshev Paul
Carlson Karen Cochrane
Genetic Therapy, Inc.
Lillie Raymond J.
Olstein Elliot M.
The United States of America as represented by the Department of
LandOfFree
Expression of human multidrug resistance genes and improved sele does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Expression of human multidrug resistance genes and improved sele, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Expression of human multidrug resistance genes and improved sele will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-883463