Nucleic acid quantitation by co-amplification of target with mul

Details Nucleic acid quantitation by co-amplification of target with mul Nucleic acid quantitation by co-amplification of target with mul

1997-09-26

1998-11-17

Jones, W. Gary

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 6, 435 911, 435 9121, 536 243, 536 2431, 536 2432, 536 2433, C12P 1934, C12Q 168, C07H 2104, C07H 2102

Patent

active

058375015

ABSTRACT:
The present invention is related to an improved method for the quantification of nucleic acid, which can be performed with a minimal amount of nucleic acid amplification reactions. The method according to the invention for the quantification of analyte nucleic acid in a sample comprises the steps of: adding to the sample different respective amounts of different nucleic acid constructs, each construct being distinguishable from the analyte nucleic acid and capable of being co-amplified with the analyte nucleic acid; subjecting the sample to a nucleic acid amplification procedure, using amplification reagents capable of reacting with both the analyte nucleic acid and the nucleic acid constructs; detecting the relative amounts of amplificates derived from analyte nucleic acid and each nucleic acid construct; calculating the amount of analyte nucleic acid from said relative amounts. Each nucleic acid construct is different; the nucleic acid constructs can be distinguished from one another and from the analyte nuclic acid. The nucleic acid constructs do resemble each other, and the analyte nucleic acid, in that all are capable of reacting with the same amplification reagents.

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