Chemistry: molecular biology and microbiology – Apparatus – Differentiated tissue perfusion or preservation apparatus
Patent
1987-05-14
1990-12-04
Kepplinger, Esther L.
Chemistry: molecular biology and microbiology
Apparatus
Differentiated tissue perfusion or preservation apparatus
435285, 435286, 435287, 435297, 435299, 435300, 435301, 43524025, 435240243, 422102, C12M 300, C12M 304, B01L 300
Patent
active
049753770
ABSTRACT:
Growth chambers for anchorage-independent cell growth therein are formed of a gel matrix having a surface disallowing anchorage-dependent cell growth over the full interior thereof. In a preferred form the chambers have a generally cylidnrical wall and an integral convex bottom wall forming an annular volume at the foot of the cylindrical wall which is substantially lower than the central portion to concentrate such anchorage-indpendent cells. The gel matrix is sufficiently permeable to permit passage of cell-growth nutrients and waste product solutes through said wall when the chambers are filled below the open end and submerged in a growth medium. Preferably the gel matrix is formed of 1% to 5% cross-linked polyacrylamide and from 99% to 95% water.
In a preferred method of using the growth chambers, undifferentiated tumor cells and normal cells, including fibroblasts, are cultured together. Anchorage-independent tumor cells proliferate while anchorage-dependent cells are unable to grow without attachment. The method is useful for evaluating in vitro therapeutic agents to control tumor growth, normal cell growth or microspheres and generation of immunoglobulins from lymphocyte cells.
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Folkman and Moscona, "Role of Cell Shape in Growth Control", vol. 273 (1 Jun. 1978), pp. 345-349.
Kepplinger Esther L.
Scheiner Toni R.
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