PCR-based cDNA substractive cloning method

Details PCR-based cDNA substractive cloning method PCR-based cDNA substractive cloning method

1997-05-19

1998-11-17

Jones, W. Gary

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

935 912, 935 76, 935 77, 935 78, C12Q 168, C12P 1934, C12N 1500

Patent

active

058374680

ABSTRACT:
A process is described in which PCR-based cDNA libraries with anchored ends are made, a PCR-based cDNA subtracted (PCSUB) library is produced therefrom, and the ends of a cDNA clone are isolated, via PCR, from an anchored-ends library.

REFERENCES:
patent: 5482845 (1996-01-01), Soares et al.
patent: 5525471 (1996-06-01), Zeng et al.
patent: 5629179 (1997-05-01), Mierendorf et al.
patent: 5643761 (1997-07-01), Fisher et al.
Frohman et al. PNAS vol. 85 : pp. 8998-9002 (1988).
Luk'Tanov et al. "Highly Efficient Subtractive Hybridization of cDNA", J. Bioorganic Chem., 20(6): 386-388 (Jan. 1, 1994).
BORSON et al., "A Lock-Docking Oligo(dT) Primer for 5' and 3+ RACE PCR", PCR Methods And Applications, vol. 2:144-148, (1992).
LISITSYN et al., "Cloning The Differences Between Two Complex Genomes", Science, vol. 259:946-951, 1993.
LIANG et al., "Distribution And Cloning of Eukaryotic mRNAs By Means of Differential Display: Refinements And Optimization", Nucleic Acids Research, vol. 21, No. 14, pp. 3269-3275, (1993).
FROHMAN, "Race: Rapid Amplification Of cDNA Ends", PCR Protocols, A Guide To Methods and Applications, pp. 28-38, (Academic Press 1990).
BELYAVSKY et al., "PCR-based cDNA Library Construction: General cDNA Libraries At The Level of a Few Cells", Nucleic Acids Research, vol. 17:2919-2932, (1989).

Affiliated with

Also associated with

Rating

PCR-based cDNA substractive cloning method does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with PCR-based cDNA substractive cloning method, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and PCR-based cDNA substractive cloning method will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-882958