Kit for obtaining a digoxin concentrate from a serum sample

Details Kit for obtaining a digoxin concentrate from a serum sample Kit for obtaining a digoxin concentrate from a serum sample

1986-10-16

1990-12-04

Hill, Jr., Robert J.

Chemical apparatus and process disinfecting, deodorizing, preser

Analyzer, structured indicator, or manipulative laboratory...

Sample mechanical transport means in or for automated...

210282, 210656, 422 70, 436 14, 436161, 436178, G01D 128

Patent

active

049752480

ABSTRACT:
Serum is passed through a column containing silica gel alkylated with lower alkyl groups. The column is then washed with dilute acid and water and the digoxin eluted with aqueous methanol at a volume less than about the volume of the serum to provide a digoxin concentrate that may be used in assay determinations. The invention also includes a kit comprising in a packaged combination for use in an assay for digoxin (a) a column containing silica gel alkylated with alkyl groups containing 1 to 2 carbon atoms and (b) an aqueous eluent solution which is 50%-70% by volume organic solvent. The organic solvent comprises from 1-4 carbon atoms and from 1-3 heteroatoms selected from the group consisting of oxygen and nitrogen.

REFERENCES:
patent: 4131544 (1978-12-01), Elahi
patent: 4409335 (1983-10-01), Hanamoto et al.
patent: 4476093 (1984-10-01), Watanabe et al.
Issaq et al., J. Liquid Chromatography, vol. 5, No. 6, pp. 1069-1080, (1982).
Issaq et al., Chemical Abstracts, vol. 97, Abstract No. 97:49014z, 1982.
Berendsen et al., J. of Chromatography, vol. 196, pp. 21-37, 1981.
Kabra et al., "Clinical Liquid Chromatography", vol. I-Analysis of Exogenous Compounds, CRC Press, 1984, 197-208.

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