Silver stains for protein in gels--a modified procedure

Details Silver stains for protein in gels--a modified procedure Silver stains for protein in gels--a modified procedure

1982-02-16

1984-08-28

Turk, Arnold

Chemistry: analytical and immunological testing

Peptide, protein or amino acid

436169, G01N 3368

Patent

active

044684665

ABSTRACT:
In a silver stain method for protein in gels utilizing treatment with a reducing agent followed by treatment with a silver salt and actuating irradiation, the improvement comprising the use of a reducing agent consisting essentially of dithiothreitol in an amount effective to stain the protein but keep background staining to a minimum.

REFERENCES:
patent: 4405720 (1983-09-01), Merril
patent: 4416998 (1983-11-01), Adams et al.
Kerenyi, et al., "A Highly Sensitive Method for Demonstrating Proteins in Electrophoretic, Immuno-electrophoretic and Immuno-diffusion Preparations", Clin. Chim. Acta, 38, 465-467 (1972).
Kerenyi, et al., "Uber Problems der Quantitiven Auswertung der mit Physikalischer Entwicklung Versilberten Agarelektrophoretogramme", Clin. Chim. Acta, 47, 425-436 (1973).
Veerheecke, "Agargel Electrophoresis of Unconcentrated Cerebrospinal Fluid", J. Neurol., 209, 59-63 (1975).
Karcher, et al., "Cerebrospinal Fluid Proteins Electrophoresis without Prior Concentration", Acta Neurol. Belg., 79, 335-337 (1979).
Switzer, et al., "A Highly Sensitive Silver Stain for Detecting Proteins and Peptides in Polyacrylamide Gels", Anal. Biochem, z8, 231-237 (1979).
Merril, et al., "Trace Polypeptides in Cellular Extracts and Human Body Fluids Detected by Two-Dimensional Electrophoresis and a Highly Sensitive Silver Stain", Proc. Natl. Acad. Sci. U.S.A., 76, 4335-4339 (1979).
Oakley, et al., "A Simplified Ultrasensitive Silver Stain for Detecting Proteins in Polyacrylamide Gels", Anal. Biochem. 105, 361-363 (1980).
Merril, et al., in Anal. Biochem., 110:201-207 (1981).
Merril, et al., in Science, 211:1437-1438 (1981).
Sun, et al., in Cell 9:511-521 (1976).

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