Method for producing a CSAp tryptic peptide and anti-CSAp antibo

Details Method for producing a CSAp tryptic peptide and anti-CSAp antibo Method for producing a CSAp tryptic peptide and anti-CSAp antibo

1981-06-01

1984-08-28

Warden, Robert J.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435184, 435272, 424 85, 424 88, 436543, C12P 2100, C12N 900, A61K 3900, A61K 39395

Patent

active

044684576

ABSTRACT:
A tryptic peptide is produced by partial tryptic digestion of purified, high molecular size colon-specific antigen-p (CSAp), to produce a lower molecular size antigen carrying the CSAp antigenic determinant. The tryptic peptide is used to produce monospecific anti-CSAp antibodies.

REFERENCES:
patent: 2719102 (1955-09-01), Baldwin
patent: 4086217 (1978-04-01), Hansen
patent: 4228236 (1980-10-01), Jakstys et al.
Sigma Chemical Co., "Biochemicals and Organic Compounds" P.O. Box 14508, St. Louis, MO 63178.
Perlmann et al., "Proteolytic Enzymes" Methods in Enzymology vol. XIX (1970) pp. 34 and 210.
Pant et al., "Further Characterization of CSAp an Antigen Associated with Gastrointestinal and Ovarian Tumors", Cancer, vol. 42 (Sep. Supplement) (9/78) pp. 1626-1634.
Shochat et al., "Isolation of Immunologically-Active Tryptic Peptides from Colon-Specific-Antigen-p (CSAp)", Proceedings American Association for Cancer Research: vol. 21 (3-1980) Abstract #886.
Beachey et al., "Repeating Covalent Structure of Streptococcal M Protein" Proceedings of the National Academy of Sciences 75(7) (1978) pp. 3163-3167.
Shochat et al., "Characterization of Colon-Specific Antigen-p and Isolation of Immunologically Active Tryptic Peptides", Journal of Immunology Vol. 126(6) (6/81) pp. 2284-2289.
Ludogovskaya et al., "Soluble Antigens of the Polyps of the Human Colon", Vop. Onkol. 13(4) (1967), pp. 39-45 Chemical Abstracts 67: 20021u.
Maisonrouge-McAuliffe et al., "Immunochemical Studies on Blood Groups", Archives of Biochemistry and Biophysics 175 pp. 71-80 (1976).
Gold et al., "Protease Digestion of Colonic Mucin", Journal of Biological Chemistry 256 (12) pp. 6354-6358 (1981).

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