Methods for gene transfer

Details Methods for gene transfer Methods for gene transfer

1996-06-17

1999-07-27

Campell, Bruce R.

Drug, bio-affecting and body treating compositions

Whole live micro-organism, cell, or virus containing

Genetically modified micro-organism, cell, or virus

435 691, 4353201, 435325, 5303896, 514 44, 424529, A01N 6300

Patent

active

059286385

ABSTRACT:
The present invention provides a method for optimizing gene transfer into hematopoietic stem cells (HSCs) by contacting the cells with hydroxyurea prior to gene transfer to induce HSCs in G0 into active cell cycle. This method is useful for treating patients suffering from a disease that is suitably treated by gene therapy or involves hematopoietic cells. A method is also provided for enhancing the efficacy of bone marrow transplantation by administering hydroxyurea to increase HSC yields.

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