Separation of high-activity heparin by affinity chromatography o

Details Separation of high-activity heparin by affinity chromatography o Separation of high-activity heparin by affinity chromatography o

1978-07-03

1979-11-20

Brown, Johnnie R.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

424183, C07H 106

Patent

active

041751825

ABSTRACT:
An affinity chromatography method for the separation of high-activity heparin from a mixture thereof with low-activity heparin. The method employs an affinity column of protamine coupled to a water-insoluble solid support material such as agarose, and a series of sodium chloride-imidazole elution buffers (pH 6.5-7.5) varying in sodium chloride molarity from about 1.3 to about 2.0. Stepwise elution of a plurality of heparin fractions from the affinity column with the elution buffers of successively increasing sodium chloride molarity results in the removal from the affinity column of first the low-activity heparin and finally the high-activity heparin.

REFERENCES:
patent: 2552507 (1951-05-01), O'Keeffe et al.
patent: 2989438 (1961-06-01), Nomine et al.
patent: 3099600 (1963-07-01), Toccaceli et al.
patent: 3451996 (1969-06-01), Sumyk et al.

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