Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase
Patent
1986-10-20
1988-12-27
Kepplinger, Esther M.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving hydrolase
435 22, 435810, C12Q 134, C12Q 140
Patent
active
047940786
ABSTRACT:
Method of measuring the amount of .alpha.-amylase in a liquid sample, including the steps of providing an oligosaccharide substrate for .alpha.-amylase, the substrate being characterized in that it contains at least 3 glucose units, its reducing-end glucose unit is bonded, via a bond cleavable by .alpha.- or .beta.-glucosidase, to a label which exhibits an optically measurable change upon cleavage of the bond, and its terminal glucose unit is bonded to a blocking substituent which inhibits cleavage by exo-enzymes of the bond between the terminal glucose unit and the adjacent glucose unit; contacting the sample with the oligosaccharide substrate and with a first exo-enzyme capable of cleaving the bond between the reducing-end glucose unit and the label, and measuring the optically measurable change as a measure of .alpha.-amylase in the sample.
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Genzyme Corporation
Kepplinger Esther M.
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