Method of making genetically stable mutants of vibrio cholerae

Details Method of making genetically stable mutants of vibrio cholerae Method of making genetically stable mutants of vibrio cholerae

1979-07-20

1981-04-28

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

435245, 435909, 424 92, C12N 1500, C12N 136, C12R 163

Patent

active

042647371

ABSTRACT:
Incubation of a parent strain of Vibrio cholerae at elevated temperature to produce a hypotoxinogenic variant strain and mutation of the variant strain results in mutant strains which retain the biotype and antigens of the parent strain, and which are genetically stable and useful as live oral vaccines for immunization against cholera. Preferably, a hypotoxinogenic mutant which is also non-pathogenic in animal model systems is isolated by the method disclosed in this application.

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