Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues
Patent
1996-12-06
1999-01-05
Ulm, John
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
435 691, 4352523, 4353201, 536 235, C07K 14705, C12N 1512
Patent
active
058564438
ABSTRACT:
The cloning and expression of two novel rat cDNAs ("H218"and "rat-edg") which encode two members ("p.sup.H218 " and "p.sup.rat-edg ") of the G-protein coupled receptor superfamily of proteins is described. The amino acid sequence similarity between "p.sup.H218 " and "p.sup.rat-edg " suggests that they may be activated by the same endogenous ligand(s). The expression pattern of mRNA transcripts of both genes in cell lines, various rat tissues and developing rat brain suggests that they both play a role in cell proliferation and/or differentiation. The polynucleotide molecules, proteins, and antibodies of the subject invention can be used in both diagnostic and therapeutic applications.
REFERENCES:
Yarden, Y. A. Ullrich (1988) "Growth Factor Receptor Tyrosine Kinases" Ann. Rev. Biochem. 57:443-478.
Devreotes, P. (1989) "Dictyostelium discoideum: A Model System for Cell-Cell Interactions in Development" Science 245:1054-1058.
Hanley, M.R. (1989) "Mitogenic neurotransmitters" Nature 340:97.
Zachary, I., P.J. Woll, E. Rozengurt (1987) "A Role for Neuropeptides in the Control of Cell Proliferation" Dev. Biol. 124:295-308.
Young, D., G. Waitches, C. Birchmeier, O. Fasano, M. Wigler (1986) "Isolation and Characterization of a New Cellular Oncogene Encoding a Protein with Multiple Potential Transmembrane Domains" Cell 45:711-719.
Gutkind, J.S., E.A. Novotny, M.R. Brann, K.C. Robbins (1991) "Muscarinic acetylcholine receptor subtypes as agonist-dependent oncogenes" Proc. Natl. Acad. Sci. USA 88:4703-4707.
Julius, D., T.J. Livelli, T.M. Jessell, R. Axel (1989) "Ectopic Expression of the Serotonin 1c Receptor and the Triggering of Malignant Transformation" Science 244:1057-1062.
Julius, D., K.N. Huang, T.J. Livelli, R. Axel, T.M. Jesell (1990) "The 5HT2 receptor defines a family of structurally distinct but functionally conserved serotonin receptors" Proc. Natl. Acad. Sci. USA 87:928-932.
MacLennan, A.J., G.D. Frantz, R.C. Weatherwax, N.J.K. Tillakaratne, A.J. Tobin (1990) "Expression of mRNAs That Encode D2 Dopamine Receptor Subtypes: Anatomical, Developmental, and Pharmacological Studies" Molec. Cell. Neurosci. 1:151-160.
Loh, E.Y., J.F. Elliott, S. Cwirla, L.L. Lanier, M.M. Davis (1989) "Polymerase Chain REaction with Single-Sided Specificity: Analysis of T Cell Receptor .delta. Chain" Science 243:217-220.
Sanger, F., S. Nicklen, A.R. Coulson (1977) "DNA sequencing with chain-terminating inhibitors" Proc. Natl. Acad. Sci. USA 74:5463-5467.
Chirgwin, J.M., E. Przbyla, R.J. MacDonald, W.J. Rutter (1979) "Isolation of Biologically Active Ribonucleic acid from Sources Enriched in Ribonuclease" Biochem. 18:5294-5299.
Okasaki et al. Biochem. and Biophys. Comm. 190(3):1104-1109, 15 Feb. 1993.
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