Human protein disulfide isomerase

Details Human protein disulfide isomerase Human protein disulfide isomerase

1998-10-27

1999-12-14

Wax, Robert A.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Isomerase

435 691, 4352523, 4353201, 530350, 536 232, C12N 990, C12N 120, C12N 1500, C07H 2104

Patent

active

060016329

ABSTRACT:
The present invention provides a polynucleotide (pdih) the partial sequence for which was initially isolated from a lung cDNA library and which identifies and encodes a novel human protein disulfide isomerase (PDIH). The invention provides for genetically engineered expression vectors and host cells comprising the nucleic acid sequence encoding PDIH. The invention also provides for the use of purified PDIH and its agonists in the commercial production of recombinant proteins and in pharmaceutical compositions for the treatment of diseases associated with the abnormal expression of PDIH. Additionally, the invention provides for the use of antisense molecules to pdih or inhibitors of PDIH in pharmaceutical compositions for treatment of diseases. The invention also describes diagnostic assays which utilize diagnostic compositions comprising the polynucleotide, fragments or the complement thereof, which hybridize with the genomic sequence or the transcript of pdih, or anti-PDIH antibodies which specifically bind to the polypeptide, PDIH.

REFERENCES:
Laboissiere, M., et al., "The Essential Function of Protein-disulfide Isomerase Is to Unscramble Non-native Disulfide Bonds," The Journal of Biological Chemistry, 270(47):28006-28009 (1995).
Tasanen, K., et al., "Promoter of the Gene for the Multifunctional Protein Disulfide Isomerase Polypeptide," The Journal of Biological Chemistry, 267 (16):11513-11519 (1992).
LaMantia, M., et al., "Glycosylation site binding protein and protein disulfide isomerase are identical and essential for cell viability in yeast," Proc. Natl. Acad. Sci. USA, 88:4453-4457 (1991).
Pihlajaniemi, T., et al., "Molecular cloning of the .beta.-subunit of human prolyl 4-hydroxylase. This subunit and protein disulphide isomerase are products of the same gene," The EMBO Journal, 6(3):643-649 (1987).
Hayano, T., et al., "Molecular cloning of the cDNA encoding a novel protein disulfide isomerase-related protein (PDIR)," FEBS Letters, 372:210-214 (1995).
Wilson, R., et al., "2.2 Mb of contiguous nucleotide sequence from chromosome III of C. elegans," Nature, 368:32-38 (1994) (GI 1086627).
Shorrosh, B., et al., "Molecular characterization and expression of an alfalfa protein with sequence similarity to mammalian ERp72, a glucose-regulated endoplasmic reticulum protein containing active site sequences of protein disulphide isomerase," The Plant Journal, 2(1)51-58 (1992) (GI 729442).
Hillier, L., et al. (GI 149007) GenBank Sequence Database (Accession N30487) National Center for Biotechnology Information, National Library of Medicine, Bethesda, Maryland 2084.
Edman, J., et al., "Sequence of protein disulphide isomerase and implications of its relationship to thioredoxin," Letters to Nature, 317:267-270 (1985).
Essex, D., et al., "Localization of Protein Disulfide Isomerase to the External Surface of the Platelet Plasma Membrane," Blood, 86(6)2168-2173 (1995).
Terada, K., et al., "Secretion, Surface Localization, Turnover, and Steady State Expression of Protein Disulfide Isomerase in Rat Hepatocytes," The Journal of Biological Chemistry, 270(35)20410-20416 (1995).
Marra, M et al., (GI 1284976) GenBank Sequence Database (Accession W10660), National Center for Biotechnology Information, National Library of Medicine, Bethesda, Maryland, 20894.
Fujiwara, T et al., (GI 968238) GenBank Sequence Database (Accession D58604), National Center for Biotechnology Information, National Library of Medicine, Bethesda, Maryland, 20894.
Ryser, HJP, et al., "Inhibition of human immunodeficiency virus infection by agents that interfere with thiol-disulfide interchange upon virus-receptor interaction" Proc. Natl. Acad. Sci USA 91:4559-4563 (1994).
Kaetzel, CS, et al., "Protein disulphide-isomerase from human placenta and rat liver: Purification and immunological characterization with monoclonal antibodies" Biochem. J. 241:39-47 (1987).
Morjana, NA et al., "Effect of Protein and Peptide Inhibitors on the Activity of Protein Disulfide Isomerase" Biochemistry 30:4985-4990 (1991).
Charnock-Jones, D., et al., Cloning, Expression and Genomic Organization of Human Placental Protein Disulfide Isomerase (Previously Identified as Phospholipase C Alpha), Int. J. Biochem. Cell Biol., 28(1): 81-89 (1996).
McLaughlin, S.H. and R.B. Freedman, "Cloning and expression of active domains of human protein disulphide isomerase", Biochemical Soc. Trans., 23: 69S (1995).
Parry, J.W.L., et al., "The expression in E. coli purification and isolated non-thioredoxin-like domains of human PDI", Biochemical Soc. Trans., 23: 71S (1995).
Vuori, K. et al.,"Expression and Site-directed Mutagenesis of Human Protein Disulfide Isomerase in Escherichia coli", J. Biol. Chem., 267(11): 7211-7214 (1992).

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