Production and use of normalized DNA libraries

Details Production and use of normalized DNA libraries Production and use of normalized DNA libraries

1998-03-04

1999-12-14

Degen, Nancy

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 912, 435440, 536 254, 536 2542, C12Q 168, C12N 1500, C07H 2100

Patent

active

060015748

ABSTRACT:
Disclosed is a process for forming a normalized genomic DNA library from an environmental sample by (a) isolating a genomic DNA population from the environmental sample; (b) at least one of (i) amplifying the copy number of the DNA population so isolated and (ii) recovering a fraction of the isolated genomic DNA having a desired characteristic; and (c) normalizing the representation of various DNAs within the genomic DNA population so as to form a normalized library of genomic DNA from the environmental sample. Also disclosed is a normalized genomic DNA library formed from an environmental sample by the process.

REFERENCES:
Simoens et al., Isolation of Genes Expressed in Specific Tissues of Arabidopsis thaliana by Differential Screening of a Genomic Library, Gene 1998, vol. 6, pp. 1-11.

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