Method of measuring concentration of substances

Details Method of measuring concentration of substances Method of measuring concentration of substances

1987-06-08

1989-05-09

Rosenberger, Richard A.

Optics: measuring and testing

For size of particles

By particle light scattering

356339, G01N 33543, G01N 2147

Patent

active

048283886

ABSTRACT:
A concentration of antigen substances contained in a sample is measured by detecting light scattered by latex particles coated with antibody substances which are specifically reactive with the antigen substances to be measured. The scattered light is received by a photodetector and an output signal of the photodetector is supplied to a fast Fourier transform unit to derive a power spectrum density of the scattered light. Then a white level of the power spectrum density is derived, and the concentration of the antigen substances in the sample is detected from the white level with the aid of a calibration curve representing a relationship between the concentration and the white level.

REFERENCES:
patent: 4139303 (1979-02-01), Carlson et al.
patent: 4259015 (1981-03-01), Wada
Howard A. Strobel: Chemical Instrumentation, Second Edition, Addison-Wesley Publishing Company, Reading, Mass., 1973, pp. 581-591.

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