Method for determining antistreptolysin O antibody

Details Method for determining antistreptolysin O antibody Method for determining antistreptolysin O antibody

1995-11-09

1999-01-05

Housel, James C.

Chemistry: analytical and immunological testing

Involving an insoluble carrier for immobilizing immunochemicals

436523, 436524, 436528, 436532, 436533, 436522, 4241651, G01N 33543

Patent

active

058562028

ABSTRACT:
A method for immunologically determining antistreptolysin O antibody comprises contacting a test sample solution containing antistreptolysin O antibody with a binding agent to selectively bind the antistreptolysin O antibody to the binding agent, and determining an amount of the bound antistreptolysin O antibody, wherein the binding agent comprises a streptolysin O-immobilized carrier obtained by contacting a solution containing streptolysin O with a carrier having immobilized thereon at least one steroid represented by formula (1): ##STR1## wherein R.sup.1 is a side chain moiety of cholesterol or a side chain moiety of cholic acid, wherein each of the side chain moieties is independently unsubstituted or substituted and independently saturated or unsaturated; R.sup.2 is hydrogen or hydroxyl; and each dashed line independently represents a single bond or no bond. The method of the present invention can be used for determining antistreptolysin O antibody specifically, accurately and easily and can be practiced not only by manual operation but also by automated operation.

REFERENCES:
R. Lancefield, "A serological differentiation of Human and Other Groups of Hemolytic Streptococci", J. Exp. Med., 1933, vol. 57, pp. 571-593.
D. Prigent et al., "Interaction of Streptolysin O with Sterols", Biochimica et Biophysica Acta, 1976, vol. 443, pp. 288-300.
T. Nagata, "Disease caused by Infection with Hemolytic Streptococcus", Rinsho-kensa (Laboratory Examinations), 1979, vol. 23, pp. 1172-1175. Translated sections only.
L. Rantz et al., "A Modification of the Technic for Determination of the Antistreptolysin Titer", Proc. soc. Exp. Biol. Med., 1945, vol. 59, pp. 22-25.
E. Edwards, "Protocol for Micro Antistreptolysin O Determinations", J. Bacteriol., 1964, vol. 87, pp. 1254-1255.
H. Fujimoto et al., "Antistreptolysin O", Rinsho-byori (Clinical Pathology), 1992, vol. 40, pp. 21-27. Eng Abst. only.
T. Miura et al., Eisei-kensa (Hygienic Examinations), 1987, vol. 36, pp. 36-40. Translated sections only.

Affiliated with

Also associated with

Rating

Method for determining antistreptolysin O antibody does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for determining antistreptolysin O antibody, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for determining antistreptolysin O antibody will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-861713