Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Patent
1991-12-13
1995-01-10
Parr, Margaret
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
435 6, 436501, 536 231, 536 241, 536 243, 536 242, 536 2431, 536 2432, 536 2433, 536 253, 536 254, 935 78, 935 88, C07H 2104, C12Q 168
Patent
active
053808339
ABSTRACT:
Novel methods for assaying a nucleic acid analyte are provided, which employ polynucleotides having oligonucleotide sequences substantially homologous to a sequence of interest in the analyte, where the presence or absence of hybridization at a predetermined stringency provides for the release of a label from a support. Particularly, various techniques are employed for binding a label to a support, whereupon cleavage of either a single or double strand, a label may be released from a support, where the release of the label can be detected as indicative of the presence of a particular oligonucleotide sequence in a sample. The method finds use in diagnosis of disease, genetic monitoring, and analysis of nucleic acid mixtures.
REFERENCES:
Maniatis et al. (1982) Molecular Cloning, A Laboratory Manual (Cold Spring Harbor Lab., CSH, New York) pp. 214-223.
Arndt-Jovin et al. (1975) Eur. J. Biochem., vol. 54, pp. 411-418.
Ashley et al. (1984) Anal. Bioch., vol. 140, pp. 95-103.
Blackburn Robert P.
Chiron Corporation
Goldman Kenneth M.
Marschel Ardin H.
Parr Margaret
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