Plant transformation method using agrobacterium species adhered

Multicellular living organisms and unmodified parts thereof and – Plant – seedling – plant seed – or plant part – per se – Multicellular algae

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4351723, A01H 104

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active

059327820

ABSTRACT:
Bacteria of the species Agrobacterium are applied to particles which are used in a typical particle gun in a manner which retains their viability after the dry-down process involved in microparticle bombardment. When plant materials are bombarded with particles coated with the bacteria, high rates of stable transformation are achieved.

REFERENCES:
patent: 4647537 (1987-03-01), Shigemitsu
patent: 4771002 (1988-09-01), Gelvin
patent: 4859377 (1989-08-01), Shasha et al.
patent: 4945050 (1990-07-01), Sanford et al.
Graves et al 1986 Plant Molec Biol 7 : 43-50.
Engstrom et al 1987. J Molec Biol 197 : 635-645.
Klein et al 1988 Bio/Technology 6 : 559-563.
Sanford 1988 trends in Biotech 6 :299-302.

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