Method for isolation of insertion elements from coryneform bacte

Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses

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43525232, 4353201, C12N 1511

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053806573

ABSTRACT:
A method locating insertion elements (IS elements) or transposons in coryneform bacteria, a positive selection system suitable for the above, the IS elements found in this manner and their use, is disclosed. The method involves:

REFERENCES:
Boehringer Mannheim GmbH Biochemica, DNA Labeling and Detection Nonradioactive, 1989.
Gay, P., et al., "Positive Selection Procedure for Entrapment of Insertion Sequence Elements in Gram-negative Bacteria", Journal of Bacteriology, Nov. 1985, pp. 918-921.
Jager, W. et al., "Expression of the Bacillus subtilis sac.beta. Gene Leads to Sucrose Sensitivity in the Gram-Positive Bacterium Corynebacterium glutamicum but Not in Streptomyces", Journal of Bacteriology, Aug., 1992, pp. 5462-5465.

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