Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1997-11-19
1999-08-03
Horlick, Kenneth R.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
435 912, 435 6, C12Q 168, C12P 1934
Patent
active
059324511
ABSTRACT:
Methods of enzymatically producing unbiased amounts of nucleic acid from mRNA are provided. In the subject methods, a sample of mRNA is converted to ds cDNA using a primer containing an RNA polymerase site and at least one priming site. The resultant ds cDNA is then asymmetrically amplified with captureable primer to produce captureable cDNA which is then converted to captured ds cDNA. The resultant ds cDNA may then be used in a number of different applications, such as in the preparation of amplified amounts of aRNA, in the preparation of cDNA probes, and the like.
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Idaho Technology, "The Rapidcycler" Idaho Technology, Inc. P.O. Box 50819, Idaho Falls, ID 83405.
Chung Alicia
Wang Bruce
Horlick Kenneth R.
Incyte Pharmaceuticals Inc.
Siew Jeffrey
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