Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1992-11-30
1995-02-28
Parr, Margaret
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 912, 536 221, 536 231, 536 243, 536 2431, 536 2433, 935 77, 935 78, 935 88, C12Q 168
Patent
active
053936574
ABSTRACT:
A method for detecting the presence of small mounts of contaminant DNA, present in an amount of at least about 0.1 picogram of DNA, in a protein product produced by recombinant DNA technology. The method comprises using oligonucleotides with sequences directed to those of repetitive host cell line DNA sequences, as primers in a PCR reaction. Also disclosed are kits for the detection of small amounts of contaminant DNA in a protein product.
REFERENCES:
Nelson et al. (1989) Proc. Natl Acad Sci (USA), vol. 86, pp. 6686-6690.
Anthony V. Cox et al., "Use of the polymerase chain reaction to detect spacer size heterogeneity in plant 5S-rRNA gene clustrs and to locate such clusters in wheat (Triticum aestivum L.)", Theor. Appl. Genet 83:684-690 (1992).
Kathleen D. Eisenach et al., "Polymerase Chain Reasction Amplification of a Repetitive DNA Sequence Specific for Mycobacterium tuberculosis" J. of Infectious Diseases 161: 977-981 (1990).
Mark Goldman et al., "Use of Polyerase Chain Reaction for Detecting DNA Contaminants in Pharmaceutical Recombinant Products" Clinical Chemistry, vol. 37, No. 9 pp. 1523-1525 (1991).
Susan R. Haynes et al., "The Chinese Hamster Alu-Equivalent Sequence: A Conserved, Highly Repetitious, Interspersed Deoxyribonucleic Acid Sequence in Mammals has a Structure Suggestive of a Transposable Element." Molecular and Cellular Biology, vol. 1, No. 7 pp. 573-583 (1981).
Walter E. Hill et al., "Polymerase Chain Reaction Identification of Vibrio vulnificus in Artificially Contaminated Oysters." Applied and Environmental Microbiology V. 57, No. 3, pp. 707-711 (1991).
Alec J. Jeffreys et al., "Hypervariable `minisatellite` Regions in Human DNA" Nature vol. 314 pp. 67-72 (Mar. 1985).
Warren R. Jelinek et al., "Repetitive Sequences in Eukaryotic DNA and Their Expression" Ann. Rev. Biochem. 51: 813-44 (1982).
Offert Landt et al., "A General Method for Rapid Site-Directed Mutagenesis using the Polymerase Chain Reaction" Gene 96: 125-128 (1990).
James R. Lupski et al., "Short, Interspersed Repetitive DNA Sequences in Prokaryotic Genomes" J. of Bacteriology vol 174, No. 14, pp. 4525-4529 (Jul. 1992).
Hideo Maki et al., "Use of Universal and Type-specific Primers in the Polymerase Chain Reaction for the Detection and Typing of Genital Human Papillomaviruses" Jpn. J. Cancer Res. vol. 82 pp. 411-419 (Apr. 1991).
Robert K. Moyzis et al., "The Distributions of Interspersed Repetitive DNA Sequences in the Human Genome"Genomics 4: pp. 273-289 (1989).
S. R. Per et al., "Quantitation of Residual Mouse DNA in Monoclonal Antibody Based Products" Symposium on Monoclonal Antibodies for Therapy, Prevention and in vivo Diagnosis of Human Disease. Utrecht, The Netherlands 1989 Develop Biol. Standard., vol. 71 pp. 173-180 (S. Karger, Basel, 1990).
Gary J. Sharples et al., "A Novel Repeated DNA Sequence Located in the Intergenic Regions of Bacterial Chromosomes" Nucleic Acids Research. vol. 18 No. 22 pp. 6503-6508 (1990).
John G. K. Williams et al., "DNA Polymorphisms Amplified by Arbitrary Primers are Useful as Genetic Markers" Nucleic Acids vol. 18 No. 22 pp. 6531-6535 (1990).
Zilla Wong et al., "Cloning a Selected Fragment From a Human DNA `Fingerprint`: Isolation of an Extremely Polymorphic Minisatellite" Nucleic Acids Research., vol. 14 No. 11 pp. 4605-4615 (1986).
Jezuit Melissa A.
Letwin Bruce W.
DesRosier Thomas J.
Genetics Institute Inc.
Lazar Steven R.
Marschel Ardin H.
Parr Margaret
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