High pH extraction composition and its use to determine a chlamy

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage

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435 721, 435 736, 435871, 435 4CM, 436825, C12Q 170, C12Q 102

Patent

active

051322056

ABSTRACT:
An extraction composition is useful for lysing chlamydial, gonococcal or herpes organisms and extracting detectable antigen from the organisms. In particular, this composition has a high pH (at least about 8) and comprises an alcoholamine which is effective in extracting antigen. It is particularly useful for extracting either or both of the lipopolysaccharide and major outer membrane protein chlamydial antigens.

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patent: 4497900 (1985-02-01), Abram et al.
patent: 4663291 (1987-05-01), Rose
patent: 4828978 (1989-05-01), Warren et al.
Caldwell et al., J. Clin. Microbiol., 18(3); pp. 539-545 (1983).
Schmeer et al., J. Clin. Microbiol., 15(5), pp. 830-834 (1982).
Terho et al., J. Immuno., 2(3&4), pp. 239-262 (1981).

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