Chemistry: molecular biology and microbiology – Differentiated tissue or organ other than blood – per se – or... – Including perfusion; composition therefor
Patent
1989-11-03
1992-07-21
Rosen, Sam
Chemistry: molecular biology and microbiology
Differentiated tissue or organ other than blood, per se, or...
Including perfusion; composition therefor
62 62, 435 2, A01N 102
Patent
active
051318504
ABSTRACT:
Disclosed herein is a method for cryopreserving musculoskeletal tissues, such as ligaments, tendons and cartilage, by placing such tissue in contact with a composition containing a cryopreserving agent comprising a cell-penetrating organic solute, which is preferably dimethylsulfoxide, and a glycosaminoglycan, which is preferably chondroitin sulphate, in an amount sufficient to cryopreserve the musculoskeletal tissue. The addition of a glycosaminoglycan to a cryopreserving agent comprising a cell-penetrating organic solute permits a broad range of cooling rates to be employed, rather than the very narrow ranges which are employed using a cryopreserving agent comprising a cell-penetrating organic solvent without the glycosaminoglycan. Also disclosed are a freezing schedule designed to maximize retention of tissue cell viability and biomechanical properties during and after the freezing process, and a thawing schedule which maximizes cell viability.
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Cryolife, Inc.
Rosen Sam
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