Method of increased production of penicillin acylase and plasmid

Details Method of increased production of penicillin acylase and plasmid Method of increased production of penicillin acylase and plasmid

1983-06-30

1985-11-19

Tanenholtz, Alvin E.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

435253, 435231, 435317, 435837, 935 29, 935 60, 935 74, 935 14, C12N 1500, C12N 120, C12N 986, C12N 100

Patent

active

045542503

ABSTRACT:
A method is provided for enhancing penicillin acylase production wherein a Bacillus subtilis plasmid containing an insert of a chromosomal DNA fragment of Bacillus megaterium which includes the appropriate gene for enhancing penicillin acylase production is cloned into Bacillus megaterium and the Bacillus megaterium containing the above plasmid is employed in the production of penicillin acylase.
A plasmid method for forming same and host plasmid combination which are useful in the above method are also provided.

REFERENCES:
patent: 3446705 (1969-05-01), Heuser et al.
Clarke et al, Methods in Enzymology, vol. 68, pp. 396-408, (1979).
Cole et al, Methods in Enzymology, vol. XLIII, pp. 698-721, (1975).
Lovett, P. S. et al., "Expression of a Foreign Procargotic Gene in Bacillus subtilis in Genetic Engineering of Microorganisms for Chemicals", (eds. Hollaender, A. et al), 51-59, (Plenum Press, New York, 1982).
Brown and Carlton, "Plasmid Mediated Transformation in Bacillus megaterium," J. Bact. 142, 508-512, (1980).
Keggins et al., "Molecular Cloning of Genetically Active Fragments of Bacillus DNA in Bacillus subtilis and Properties of Vector Plasmid pUB110," Proc. Nat. Acad. Sci. USA 75, (1978).

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