Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1985-01-04
1989-11-07
Weimar, Elizabeth C.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
4352521, 435822, C12P 1904, C12N 120, C12R 101
Patent
active
048792284
ABSTRACT:
A water-soluble levan having a weight average molecular weight of about 10,0000-40 million, preferably about 5-25 million and especially about 10-20 million which stabilizes a bovine serum albumin colloid having index (EAI) of about 3-100 determined according to the formula EAI=2T/OC wherein T is turbidity measured at 500 nm, C is the weight of emulsified protein per unit volume of the aqueous phase, and O is the volume fraction of a dispersed oil phase is produced by fermenting a nutrient growth medium feedstock having a carbon source consisting essentially of an assimilable sugar selected from the group consisting of sucrose, raffinose, or a mixture thereof with a Zymomonas mobilis microorganism capable of converting at least 50% of the fructose value of the sugar to said levan under nutrient growth conditions which enhance levan production while suppressing ethanol production. It is useful as a colloid stabilizing agent, particularly with foods, beverages pharmaceuticals, dentrifices, and cosmetics.
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Swings et al., "The Biology of Zymomonas"; Bact. Rev., vol. 41, No. 1, (1977); pp. 1-46 (p. 29 in particular).
Park et al.; "Study On Levan Formation During Fermentation of Zymomonas mobilis on Sucrose"; Biotech. Lett., vol. 5, No. 8 (1983) pp. 515-518.
Viikari, L., "Formation of Levan and Sorbitol from Sucrose by Zymomonas mobilis"; Appl. Microbiol. Biotechnol., vol. 19 (Apr. 1984); pp. 252-255.
Dally, Ellen Lee, "Plasmid Studies in Zymomonas mobilis" Master's thesis, Rutgers State University of New Jersey (1982).
Dally Ellen L.
Mays Thomas D.
IGI Biotechnology, Inc.
Weimar Elizabeth C.
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