Novel NAD synthetase, assay method using said novel NAD syntheta

Details Novel NAD synthetase, assay method using said novel NAD syntheta Novel NAD synthetase, assay method using said novel NAD syntheta

1987-09-10

1990-05-01

Rosen, Sam

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

435 15, 435 16, 435 17, 435 21, 435 25, 435 26, 435 27, 435 28, 435183, C12Q 100

Patent

active

049217860

ABSTRACT:
A novel NAD synthetase is produced by culturing a broth of Bacillus stearothermophilus H-804 FERM BP-1408. This new enzyme selectively catalyzes the reaction ##STR1## without catalyzing the reaction ##STR2## The enzyme uses ammonia or ammonium ion as a substrate, but does not use either glutamine or asparagine. Also disclosed is an assay method using the enzyme, for any one of ATP, deamide-NAD, ammonia or ammonium ion in a specimen to be assayed.

REFERENCES:
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"Isolation of Different Thermophilic Enzymes from Bacillus stearothermophilus", Experientia, vol. 29, No. 8, Aug. 1973, By. H. Hengartner et al., pp. 941-942.
"Biosynthesis of Diphosphopyridine Nucleotide", Journal of Biological Chemistry, vol. 233, No. 2, 1958, by J. Preiss et al., pp. 493-500.
"Biosynthesis of Diphosphopyridine Nucleotide", Journal of Biological Chemistry, vol. 236, No. 2, 1960, By John Imsande et al., pp. 525-530.
"Purification and Properties of Yeast Nicotinamide Adenine Dinucleotide Synthetase", Journal of Biological Chemistry, vol. 247, No. 15, 1972, By Cheng Kai Yu et al., pp. 4794-4802.
"Pathway of Diphosphopyridine Nucleotide Biosynthesis in Escherichia coli", Journal of Biological Chemistry, vol. 236, No. 5, 1961, By John Imsande, pp. 1494-1497.

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