Fluorescent enzyme substrates

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

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549214, 549283, C07H 1704, C07D31102

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active

059985933

ABSTRACT:
Fluorescent enzyme substrates are provided. The substrates are derivatives of coumarin having an enzyme cleavable group at position 7 and a hydrophilic amide group at position 4. The compounds are highly water soluble, stable at ambient and reduced temperatures and fluoresce upon cleavage by an enzyme.

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Adamczyk, M., et al., "Synthesis of 7-Hydroxy-4-(.omega.-Carboxyalkyl)coumarins and 7-(Dimethylamino)-4-(.omega.-Carboxyalkyl)Courmarins", OPPI Briefs, 28(5):627-633 (1996).
Baggett, N., et al., "7-Hydroxycoumarin-4-acethydrazide: A fluorescent derivatizing reagent for aldehydes and ketones", Analytica Chimica Acta, 265:111-115 (1992).
Baggett, N., et al., "7-.beta.-D-Galactopyranosyloxycoumarin-4-acetic acid and its methyl ester as substrates for the .beta.-D-galactosidase of Escherichia coli", Carbohydrate Research, 197:295-301 (1990).
Baker, W., et al., "Fluorescent Reagents. Acyl Chlorides and Acyl Hydrazides", The University of Bristol, 170-173 (1949).
Gayo, L., M., et al., "Use of Pentafluorophenyl Esters for One-Pot Protection/Activation of Amino and Thiol Carboxylic Acids", Pergamon, 4915-4918 (1996).

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