Method to isolate clusters of cell subtypes from organs

Details Method to isolate clusters of cell subtypes from organs Method to isolate clusters of cell subtypes from organs

1987-06-08

1992-01-07

Weimar, Elizabeth C.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

4352401, 435268, 43524021, 435283, 435287, C12N 500, C07G 1500, A01N 102, C12M 100

Patent

active

050791605

ABSTRACT:
A method to obtain purified, well-defined cell populations from intact organs uses digestion of the distended organ with suitable proteolytic enzymes and harvest of the cell subpopulation by screening the effluent from treatment of the organ with physiologically compatible medium by a filtration screen permitting the passage of the desired cells or clusters of cells, but preventing the passage of larger particles. In this manner, physical/mechanical disruption of the cell population is unnecessary, and the cells or clusters are eased out of their structural matrix and harvested directly. Application of this method to the preparation of purified Islets of Langerhans from intact pancreas is described.

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