Polate competitive protein binding assay

Details Polate competitive protein binding assay Polate competitive protein binding assay

1981-07-30

1983-08-16

Nucker, Christine M.

Chemistry: analytical and immunological testing

Biospecific ligand binding assay

Utilizing isolate of tissue or organ as binding agent

422 61, 436804, 436808, 436542, G01N 3354, G01N 3356, G01N 3358

Patent

active

043992285

ABSTRACT:
Improvement in a competitive protein binding assay for N-5-methyltetrahydrofolic acid wherein any folate standard used is N-5-methyltetrahydrofolic acid complexed with folate binder protein. The protein of the complex is destroyed prior to the addition of immobilized folate binder protein. The immobilized folate binder protein participates in competitive binding with unknown folate and labeled folate reagents.

REFERENCES:
patent: 4146602 (1979-03-01), Gutcho et al.
patent: 4336185 (1982-06-01), Niswender
patent: 4350659 (1982-09-01), Riceberg
Kamen et al., J. Lab. Clin. Med, 83:164-174 (1974).
Theobald et al., Clin. Chem. 27:553-555 (1981).

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