Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Patent
1981-01-29
1983-08-16
Warden, Robert J.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
435 29, 435 32, 435 39, 435291, 435808, C12Q 104, C12Q 118, C12Q 106, C12M 134
Patent
active
043992196
ABSTRACT:
Molecules or cells of materials exhibiting a microbiological activity, suppression or interaction of interest are isolated from a large population of similar molecules, cells or the like. The activity of the microbiologically active molecules or living matter of interest is measured by suspending the population in a liquid medium capable of forming a gel. The resultant suspension is formed into droplets which are caused to gel. The gel droplets (GMDs) are treated to effect desirable alteration of the microbiologically active material and the amount of metabolites or reaction products of the desired alteration within each of the gel droplets. Alternatively, incubation is carried out such that each GMD initially containing one cell contains many, a microcolony, which can be tested for desirable properties while retaining sufficient viable cells for further growth and harvesting for further tesing and/or production. The gel droplets are then separated based upon the measured or sensed characteristic, metabolite or reaction product.
REFERENCES:
patent: 3560754 (1971-02-01), Kamentsky
patent: 3710933 (1973-01-01), Fulwyler et al.
patent: 3790492 (1974-02-01), Fulwyler
patent: 4162282 (1979-07-01), Fulwyler et al.
patent: 4242447 (1980-12-01), Findl et al.
Cook Paul J.
Engellenner Thomas J.
Massachusetts Institute of Technology
Smith, Jr. Arthur A.
Warden Robert J.
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