Method and reagent for the determination of glycerin

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving oxidoreductase

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435 19, 435 27, 435 28, 435190, 435254, 435805, 435810, 435815, 435913, 435816, C12Q 126, C12Q 128, C12Q 130, C12Q 144, C12N 114, C12N 904, C12R 166

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043992188

ABSTRACT:
For the determination of glycerin by oxidation with oxygen in the presence of glycerinoxidase and measurement of the oxygen consumption or of the H.sub.2 O.sub.2 formation, a glycerinoxidase from Aspergillus spec. DSM 1729 is used. A reagent suitable for this method consists of glycerinoxidase from Aspergillus spec. DSM 1729 and a system for the determination of H.sub.2 O.sub.2 and contains additionally, if desired, an agent for the saponification of esterified glycerin.

REFERENCES:
patent: 4098574 (1978-07-01), Dappen
patent: 4202941 (1980-05-01), Terada et al.
patent: 4212938 (1980-07-01), Gruber et al.
patent: 4223090 (1980-09-01), Mazza
patent: 4255519 (1981-03-01), Terada et al.
patent: 4266023 (1981-05-01), Terada et al.

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