Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1986-04-21
1989-06-27
Warren, Charles F.
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
435 68, 435320, 536 27, 935 29, 935 41, 935 47, C12N 1500, C12P 2100, C07H 1512
Patent
active
048430064
ABSTRACT:
This invention relates to the expression and accumulation in bacterial cells of heterologous polypeptides under the control of the recA promoter/operator region. One such polypeptide comprises a recA/somatostatin fusion polypeptide. This fusion polypeptide has been expressed and accumulated by E. coli with much greater effectiveness than other somatostatin-containing fusion polypeptides. In addition, use of the recA promoter/opeerator region provides greater induction flexibility than other inducible promoter systems.
REFERENCES:
patent: 4366246 (1982-12-01), Riggs
Miki et al. 1981 "Construction of a Fused Operon Consisting of the recA and Kan(Kanamycin Resistance) Genes and Regulation of its Expression . . . " Mol. Gen. Genet. v 183, pp. 25-31.
Feinstein et al 1983 "Expression of Human Interferon Genes using the recA promoter of E.coli" Nucleic Acids Research v11(9) 2927-2941.
Casaregola et al 1982 "Quantitative Expression of recA Gene Expression in E. coli" Mol Gen Genet v 185 430-39.
Eitner et al., 1982 "Interspecies recA Protein Substitution in E. coli and Proteus mirabilis" Mol Gen Gen v 185 481-86.
Beck et al 1982 "Nucleotide Sequence and Exact Localization of the Neomycin Phosphotransferase Gene from Tn5" Gene v19 527-36.
Fox David T.
Hoerner, Jr. D. R.
Monsanto Company
Swaney L. R.
Warren Charles F.
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