Method of staining microscopic organisms

Details Method of staining microscopic organisms Method of staining microscopic organisms

1976-06-30

1978-06-13

Naff, David M.

Textiles: fiber preparation

424 7, C12K 104

Patent

active

040947459

ABSTRACT:
A rapid method of staining microorganisms with fluorochrome dyes to render them readily visible when illuminated with U.V. radiation and viewed under a microscope is described. The method involves chemical treatment of a sample containing microorganisms suspended in a liquid medium with phosphate ions to modify (chemically) the dye-receptor sites in the microorganisms and then staining the treated microorganisms with a fluorochrome dye. Suitable additional treatments which enhance fluorescence are also possible, including methylation, esterification, hydrolysis, oxidation and treatment with sulphur dioxide. The method is particularly advantageous in the microscopic enumeration of microorganisms. Also certain techniques are described which permit the determination of viable and non-viable cells.

REFERENCES:
patent: 3670072 (1972-06-01), Mauthner
patent: 3678151 (1972-07-01), Horonick et al.
patent: 3790447 (1974-02-01), Hirata et al.
Lamanna, et al., Basic Bacteriology, The Williams and Wilkins Co., Baltimore, Md., 1965, 3rd ed. (pp. 108-110, 125-127, 134, 136, 137, 146, 147, 151, 152 & 154-159).

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