Optics: measuring and testing – By polarized light examination – With light attenuation
Patent
1992-02-28
1994-03-29
Rosenberger, Richard A.
Optics: measuring and testing
By polarized light examination
With light attenuation
356243, 356435, 356320, 356326, 250577, G01B 1128, G01N 2100
Patent
active
052989780
ABSTRACT:
Methods and apparatus for accurately determining the volume of a pipette using a photometer. A reagent system for use with the photometer includes a first container holding a reference solution and at least one second container holding a sample solution. A container holding the reference solution having maximum absorbance at a first wavelength is positioned in the photometer. The absorbances of the container at the first wavelength and at a second wavelength are measured. Then the optical pathlength of the container at the second wavelength is calculated from the measured absorbances at the first and second wavelengths. An aliquot of the sample solution is introduced into the container with a pipette to be calibrated. The sample solution has maximum absorbance at the second wavelength. The sample solution is mixed with the reference solution to form a mixture. Then the absorbance of the mixture in the container is measured at the second wavelength. The volume of the aliquot is calculated from the absorbance of the mixture and the optical pathlength of the container. The volume of the aliquot represents the volume of the pipette.
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Cohn et al., "Precision Techniques For Measuring Liquid Quantity," Control Engineering, vol. 15, Jan. 1968, U.S., pp. 51-55.
Curtis Richard H.
Rundell Ann E.
Artel, Inc.
Pham Hoa Q.
Rosenberger Richard A.
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