Method for making nucleic acid probes

Details Method for making nucleic acid probes Method for making nucleic acid probes

1988-05-05

1990-03-20

Rosen, Sam

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

536 24, 536 26, 536 27, 435 6, C07H 1900, C07H 2100

Patent

active

049103008

ABSTRACT:
Modified nucleotides are provided which have the structure ##STR1## wherein R.sup.1 is a reactive group derivatizabale with a detectable label, R.sup.2 is an optional linking moiety including an amide, thioether or disulfide linkage or a combination thereof. R.sup.3 is hydrogen, methyl, bromine, fluorine or iodine, R.sup.4 is hydrogen, an acid-sensitive, base-stable blocking group or an acyl capping group, R.sup.5 is hydrogen or a phosphorus derivative, R.sup.6 is H, OH, or OR where R is a protecting group and x is an integer in the range of 1 and 8 inclusive. Methods of synthesizing the derivatizable nucleotide are disclosed, as are labeled polynucleotide probes prepared therefrom.

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