Sequencing DNA; a modification of the polymerase chain reaction

Details Sequencing DNA; a modification of the polymerase chain reaction Sequencing DNA; a modification of the polymerase chain reaction

1988-06-01

1998-10-06

Wilson, James O.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

536 254, 536 2541, 435 6, 435 911, C07H 2100, C12Q 168, C12P 1934

Patent

active

058177973

ABSTRACT:
The invention provides a process wherein a biotinylated oligonucleotide primer and an oligonucleotide primer which has not undergone biotinylation are used when amplifying a DNA sequence to facilitate separation of the DNA strands following the polymerase chain reaction process. The biotinylation/PCR product is then exposed to a support which will selectively bind the biotinylated strand to allow selective elution of the product.

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