Gene and process for producing a thermostable urease

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

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4351723, 435227, 435228, 43525233, 536 232, 536 237, C12N 1557, C12N 1531, C12N 1511, C12P 2100

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052983994

ABSTRACT:
An isolated structural Bacillus sp. TB-90 (FERM BP-795) urease gene, which comprises base sequences encoding the amino acid sequences of three subunits of urease. A recombinant DNA comprising Bacillus sp. TB-90 (FERM BP-795) urease gene capable of replicating in Escherichia coli. A process for producing urease, which comprises cultivating Escherichia coli carrying a recombinant DNA comprising Bacillus sp. TB-90 (FERM BP-795) urease gene capable of replicating in Escherichia coli and recovering urease from the culture mixture

REFERENCES:
patent: 4753882 (1988-06-01), Takashio et al.
Harry L. T. Mobley et al, "Microbial Ureases: Significance, Regulation, and Molecular Characterization", Microbiological Reviews, vol. 53, 85-108, Mar. 1989.
Bradley D. Jones et al, "Proteus mirabilis Urease: Nucleotide Sequence Determination and Comparison with Jack Bean Urease", Journal of Bacteriology, vol. 171, No. 12, 6414-6422, 1989.
Gerhard Morsdorf et al, "Cloning of the genes encoding urease from Proteus vulgaris and sequencing of the structural genes", FEMS Microbiology Letters, 67-74, 1990, vol. 66.
Scott B. Mulrooney, "Sequence of the Klebsiella aerogenes Urease Genes and Evidence for Accessory Proteins Facilitating Nickel Incorporation", Journal of Bacteriology, vol. 172, No. 10, 5837-5843, 1990.
Kim et al., Kor. J. Appl. Microbiol. Bioeng. 13(3):297-302, 1985; Biol Abstr 81(3):AB-393, Ref. No. 23,024, Feb. 1, 1986.
Jones et al., "Proteus mirabilis Urease . . . ", J. Bacter. 170:3342-3349, Aug. 1988.

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