Receptor-tyrosine kinase assay in yeast

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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45 71, 451723, C12N 1581

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active

059552883

ABSTRACT:
Disclosed herein are compositions and methods which are useful in the identification and isolation of components involved in transmembrane receptor-mediated signaling. Such components include the receptors themselves (e.g., tyrosine kinase receptors, cytokine receptors and tyrosine phosphatase receptors), as well as ligands which bind the receptors and modulators of the downstream intracellular catalytic event which characterizes receptor-mediated signalling.

REFERENCES:
King et al., "Control of Yeast Mating Signal Transduction by a Mammalian beta2-Adrenergic Receptor and GS alpha subunit", Science 250:121-123, Oct. 5, 1990.
Miki et al. "Expression cDNA Cloning of the KGF Receptor by Creation of a Transforming Autocrine Loop", Science 251:72-75, Jan. 4, 1991.
Florio et al., Molecular Biology of the Cell 5: 283 (1994).
Keating and Williams, Science 239: 914 (1988).

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