Oligonucleotides for detection of baculovirus infection

Details Oligonucleotides for detection of baculovirus infection Oligonucleotides for detection of baculovirus infection

1994-11-22

1996-05-28

Jones, W. Gary

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

536 2432, 435 6, 435 912, C07H 2104, C12P 1934, C12Q 168

Patent

active

055212999

ABSTRACT:
Two pairs of PCR primer mixtures to be used to detect baculovirus infection: (i) a mixture of 25 to 35-base oligonucleotides including, respectively, 5'-TTTTGACGCAAATYYTAGACGCCGT-3' (SEQ ID NO: 1), and a mixture of 19 to 29-base oligonucleotides, including, respectively, 5'-TCARYATKGATTGAATWTC-3' (SEQ ID NO: 2); and (ii) a mixture of 30 to 40-base oligonucleotides, including, respectively, 5'-TAYGTGTACGACAACAARTAY-3' (SEQ ID NO: 3), and a mixture of 30 to 40-base oligonucleotides, including, respectively, 5'-GCGTCKGGYGCAAAYTCYTTWACY-3' (SEQ ID NO: 7).

REFERENCES:
patent: 4683202 (1987-07-01), Mullis
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Iatrou et al., "Polyhedrin Gene of Bombyx mori Nuclear Polyhedrosis Virus,", Journal of Virology 54:436-445, 1985.
Hooft Van Iddekinge et al., "Nucleotide Sequence of the Polyhedrin Gene of Autographa californica Nuclear Polyhedrosis Virus," Virology 131:561-565, 1983.
Kuzio et al., "Nucleotide Sequence of the p10 Polypeptide Gene of Autographa californica Nuclear Polyhedrosis Virus," Virology 139:414-418, 1984.
Leisy et al., "Nucleotide Sequencing and Transcriptional Mapping of the Orgyia pseudotsugata Multicapsid Nuclear Polyhedrosis Virus p10 Gene," Virology 153:157-167, 1986.
Leisy et al., "Location and Nucleotide Sequence of the Orgyia pseudotsugata Single Nucleocapsid Nuclear Polyhedrosis Virus Polyhedrin Gene," The Journal of General Virology, 67:1073-1079, 1986.
Leisy et al., "The Nucleotide Sequence of the Polyhedrin Gene Region from the Multicapsid Baculovirus of Orgyia pseudotsugata," Virology vol. 153:280-288, 1986.
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