Optimized catalytic DNA-cleaving ribozymes

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

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536 231, 536 245, 435 6, 435 9131, 4351721, 4351723, C12N 922, C12Q 168

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active

055809677

ABSTRACT:
The present invention discloses nucleic acid enzymes capable of cleaving nucleic acid molecules, including single-stranded DNA, in a site-specific manner under physiologic conditions, as well as compositions including same. The present invention also discloses methods of making and using the disclosed enzymes and compositions.

REFERENCES:
Joyce, Pure & Appl. Chem 65 (1993), pp. 1205-1212.
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Green, et al., "In Vitro Genetic Analysis of the Tetrahymena Self-Splicing Intron", Nature 347: 406-408 (1990).
Chowrira, et al., "Novel Guanosine Requirement for Catalysis for the Hairpin Ribozyme", Nature 354: 320-322 (1991).
Joyce, "Amplification, Mutation and Selection of Catalytic RNA", Gene 82: 83-87 (1989).
Beaudry and Joyce, "Directed Evolution of an RNA Enzyme", Science 257: 635-641 (1992).
Robertson and Joyce, "Selection In Vitro of an RNA Enzyme that Specifically Cleaves Single-Stranded DNA", Nature 344: 467-468 (1990).
Tuerk, et al., "Systematic Evolution of Ligands by Exponential Enrichment: RNA Ligands to Bacteriophage T4 DNA Polymerase", Science 249: 505-510 (1990).
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Zaug and Cech, "The Intervening Sequence RNA of Tetrahymena is an Enzyme", Science 231: 470-475 (1986).
Inoue, et al., "New Reactions of the Ribosomal RNA Precursor of Tetrahymena and the Mechanism of Self-Splicing", J. Mol. Biol. 189: 143-165 (1986).
Joyce, et al., "Catalytic Activity is Retained in the Tetrahymena Group I Intron Despite Removal of the Large Extension of Element P5", Nucl. Acids Res. 17: 7879-7889 (1989).
Cadwell and Joyce, "Randomization of Genes by PCR Mutagenesis", PCR Methods Appl. 2: 28-33 (1992).
Lehman & Joyce, "Evolution In Vitro of an RNA Enzyme With Altered Metal Dependence", Nature 361: 182-185 (1993).
Joyce and Inoue, "A Novel Technique for the Rapid Preparation of Mutant RNAs", Nucl. Acids Res. 17: 711-722 (1989).

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