Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1994-08-19
1996-12-03
Jones, W. Gary
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 15, 435 18, 435 911, 435 912, 536 2433, 536 266, 935 77, 935 78, C07H 2104, C12P 1934, C12Q 168
Patent
active
055807305
ABSTRACT:
Qualitative and quantitative methods for detecting the presence of double-stranded, non-5'-phosphorylated DNA in samples that may also contain 5'-phosphorylated DNA and/or single-stranded DNA are described. These methods involve treating the sample with an enzyme that specifically degrades 5'-phosphorylated DNA together with an enzyme that specifically degrades single-stranded DNA. More specifically, methods are described for the detection of the products of DNA amplification reactions, such as the polymerase chain reaction (PCR), wherein post-amplification enzyme digestion substantially reduces or eliminates background signals that are apparently caused by the presence of template DNA and primers in the sample after amplification is complete.
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Jones W. Gary
Olympus America Inc.
Tran Paul B.
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