Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1994-02-02
1996-12-03
Jones, W. Gary
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 691, 435 711, 435 71, 436501, 5303871, 5303881, 530399, 530806, 530808, 935 10, 935 11, 935 12, 935 13, 935 14, 935 15, 935 76, 935 77, 935 82, C12Q 168, G01N 33566, C07K 1600, C12N 1500
Patent
active
055807232
ABSTRACT:
The invention provides methods for the systematic analysis of the structure and function of polypeptides by identifying active domains which influence the activity of the polypeptide with a target substance. Such active domains are determined by substituting selected amino acid segments of the polypeptide with an analogous polypeptide segment from an analog to the polypeptide. The analog has a different activity with the target substance as compared to the parent polypeptide. The activities of the segment-substituted polypeptides are compared to the same activity for the parent polypeptide for the target. A comparison of such activities provides an indication of the location of the active domain in the parent polypeptide. The invention also provides methods for identifying the active amino acid residues within the active domain of the parent polypeptide. The method comprises substituting a scanning amino acid for one of the amino acid residues within the active domain of the parent polypeptide and assaying the residue-substituted polypeptide so formed with a target substance. The invention further provides polypeptide variants comprising segment-substituted and residue-substituted growth hormones, prolactins and placental lactogens.
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Cunningham Brian C.
Wells James A.
Genetech, Inc.
Haliday Emily M.
Jones W. Gary
Sisson Bradley L.
Terlizzi Laura
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