Process for the production of succinic acid by anaerobic ferment

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing oxygen-containing organic compound

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4352521, 435170, 435822, 562593, 562590, C12P 746, C12P 104, C12N 120, C07C 5142

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051438330

ABSTRACT:
A method of producing succinic acid comprises growing a succinate producing microorganism in medium containing sodium ions an assimilable carbohydrate, other required nutrients and dissolved carbon dioxide at pH of about 5.8 to about 6.4. The succinic acid can be isolated from the fermentation broth if it is present in high enough concentrations by maintaining the pH at about 5.8 to about 6.4 and adding calcium hydroxide so that calcium succinate precipitates.

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Gerhadt et al., Manuel of Methods for General Bacteriology, ASM, 1981, pp. 67-69.
Boyer, R, Modern Experimental Biochemistry, 1985, pp. 80-82.
Bergey's Manual of Systematic Bacteriology, Krieg et al. ed, 1984, pp. 604-605.
Hopgood et al., Aust. J. Biol. Sci., vol. 20, pp. 165-192 (1967).
Caldwell et al., J. Bacteriol., vol. 98, pp. 668-676 (1969).
Caspari et al., Arch Microbiol., vol. 135, pp. 16-24 (1983).
Davis et al., Int. J. Syst. Bacteriol., vol. 26, pp. 498-504 (1976).

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