Immunoassay method for measuring immunological antigen in fluid

Details Immunoassay method for measuring immunological antigen in fluid Immunoassay method for measuring immunological antigen in fluid

1983-08-03

1986-09-23

Nucker, Christine M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

422 56, 422 57, 422 58, 436514, 436515, 436535, 436807, 435805, C12Q 100, G01N 2164, G01N 33558, G01N 33543

Patent

active

046135677

ABSTRACT:
There is disclosed an immunoassay comprising carrying out an immunological reaction by contacting a fluid sample with an element for immunoassay, having at least three layers of a first detecting layer, a blocking layer and a second detecting layer which are successively laminated, the element containing a substance capable of binding specifically to an immunologically active substance in a fluid sample only in either one of the first and the second detecting layers, and then measuring the detected quantities corresponding to the immunological reaction quantities from both of the first and the second detecting layers.
The immunoassay by the use of the immunological analytical element according to this invention improves the precision immunoassay by measuring both of Bound and Free.

REFERENCES:
patent: Re30267 (1980-05-01), Bruschi
patent: 4255384 (1981-03-01), Kitajima et al.
patent: 4294817 (1981-10-01), Burgett et al.
patent: 4363874 (1982-12-01), Greenquist
patent: 4390343 (1983-06-01), Walter
patent: 4459358 (1984-07-01), Berke
patent: 4472498 (1984-09-01), Masuda et al.

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