Method for cloning and producing the SFII restriction endonuclea

Details Method for cloning and producing the SFII restriction endonuclea Method for cloning and producing the SFII restriction endonuclea

1993-04-21

1997-06-10

Fleisher, Mindy B.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

435 6, 4351723, 4352523, 435243, 4353201, 336 232, 935 1, 935 6, 935 22, 935 33, 935 52, 935 66, C12P 2100, C12N 1563, C12N 510, C07H 2102

Patent

active

056374763

ABSTRACT:
The present invention is directed to a method for cloning and producing the SfiI restriction endonuclease by 1) introducing the restriction endonuclease gene from S. fimbriatus into a host whereby the restriction gene is expressed; 2) fermenting the host which contains the plasmid encoding and expressing the SfiI restriction endonuclease activity; and 3) purifying the SfiI restriction endonuclease from the fermented host which contains the plasmid encoding and expressing the SfiI restriction endonuclease activity.

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